Radio host Jackie O has filed legal proceedings against radio network ARN, claiming compensation of at least $82.25 million and alleging that her termination was against the Fair Work Act.

In a notice to shareholders this morning, ARN said it disputed Jackie “O” Henderson’s claims and will defend against the suit in court.

It is the latest salvo in the ongoing feud between the network and its once highest-rating program, the Kyle and Jackie O show.

Jackie ‘O’ Henderson has launched legal action. (WireImage)

The announcement comes after Henderson’s long-time colleague, Kyle Sandilands, launched his own lawsuit against ARN after his contract was terminated.

The duo, who dominated Sydney radio ratings for years, saw their audience fall in a much-touted move to Melbourne more recently.

The co-hosts fell out after an on-air argument on February 20, after which Henderson reportedly declared she was unable to work with Sandilands.

The co-hosts fell out after an on-air argument on February 20. (Nine)

Both of their contracts were ultimately torn up and the show was cancelled.

In its statement to shareholders, ARN said Henderson also accused the company of making “misleading and deceptive” market statements, a claim ARN also denies.

The proceedings have been filed in the Federal Court, where Sandilands’ own lawsuit against his former employer was first heard last Friday.

Sandilands revealed his contract had been terminated on March 18, less than an hour before ARN Media confirmed the news in a market announcement published on the Australian Stock Exchange.

Sandilands and Henderson have been on air together for more than 25 years. (Instagram/Jackie O)

“ARN Media advises that it has issued a notice of termination of contract to Mr Kyle Sandilands and Quasar Media Services Pty Ltd,” the statement said.

“As a result, the Kyle and Jackie O show will no longer be presented.”

Sandilands said he had apologised to Henderson the night after the on-air argument but claimed ARN had used the rift to “burn the place down”.

Details of the on-air blow-up that derailed Kyle and Jackie O’s $200 million deal

The on-air fight began after Jackie O read from Andrew Mountbatten-Windsor’s astrology birth chart and Sandilands complained about her interest in astrology charts.

“Your fixation on this has made you almost unworkable,” Sandilands told her.

“You are off with the fairies with this s—, it’s mental.”